2013-2014

Nikos Fatsis Kavalopoulos
linkedin-button.png

the following is a brief synopsis of the thesis, that can be found here
thesis page in N.T.U.A DSpace


Introduction


The increasing need to implement and execute a large variety of biochemical assays, in order to detect t proteins has led to the development of a great number of automated devices with the ability to execute complex biochemical reactions. The use of microfabricated devices is ideal for this kind of applications, because they present a lot of flexibility in their use and better results throughout. In this diploma thesis the most popular assays and devices where studied and compared in order to find the most suited biological assay and the best characteristics of a microdevice. A microfluidic lab-on-a-chip microanalalysis system was designed and fabricated capable of executing a large amount of biological assays specifically ELISA for the detection of proteins. Methods of passive fluid handling where designed and implemented, superhydrophobic microvalves and UV activated shut-off valves, in order for the final microfluidic chip to be as autonomous and energy efficient as possible. The passive control and the design of the chip make it a point of care system, capable of operating outside a laboratorial environment. A large variety of different imprint and sealing methods where studied and executed, under the process of finding a reliable and efficient solution to make a large number of microfluidic chips to be the core of a point-of-care micro-total-analysis system to detect proteins



Design of the microfluidic chip

kavalothesis1.JPG

kavalothesis2.JPG



experimental and analytical models for the calculation of reagent dispersion

kavalothesis3.JPG
kavalothesis4.JPG



kavalothesis5.JPG


Final design

kavalothesis6.JPG