Abstract

Cells are secreting proteins in order to communicate. A common technique that is used for measuring proteins is ELISA, in which proteins are immobilized on a surface using biomolecules of affinity. Through this process biological fluids can be measured such as blood, plasma and supernatant from cell cultures, in a large distance from the source of secretion, which is the surface of the cells. To better study cell signaling, a combination of methods must be performed, for measuring proteins near and far from the source of secretion. The scope of this thesis is to develop a method, in which proteins can be measured near the cell surface.

The thesis can be found in here